A research group discovers a new version of the enzyme happyase that catalyzes the chemical reaction: HAPPY to/from SAD. The researchers begin to characterize the enzyme.
A.) In he first experiment, with [Etot] at 4nM, they find that the Vmax is 1.6 uMs-1. Based on this experiment, what is the Kcat for happyase? (include appropriate units)
B.) In another experimeent, with [Etot] at 1 nM and [HAPPY] at 30 uM, the researchers find that V0 = 300 nM s-1. What is the measured Km of happyase for its substrate HAPPY? (include appropriate units)